| Achaearanea maricaoensis Elizabeth Ba..., 1942 | Cobweb spiders |
| Acragas peckhami Chickering, 1946 | Jumping spiders |
| Agyneta cincta Millidge, 1991 | |
| Agyneta straminicola Millidge, 1991 | |
| Akela charlottae Peckham & Pe..., 1896 | Jumping spiders |
| Albionella propria Arthur Merto..., 1946 | |
| Allocosa senex Mello-Leitao, 1945 | |
| Alpaida tuonabo Chamberlin &..., 1936 | Orb weavers |
| Anapis keyserlingi Gertsch, 1941 | |
| Anapistula secreta Gertsch, 1941 | Minute litter spiders |
| Anaptomecus longiventris Eugène Simon, 1903 | Huntsman spiders |
| Anasaitis canalis Chamberlin, 1925 | Jumping spiders |
| Anasaitis canosa Walckenaer, 1837 | Twin-flagged jumping spider |
| Ancylometes bogotensis Eugen von Ke..., 1877 | Wandering spiders |
| Anelosimus baeza Agnarsson, 2006 | Cobweb spiders |
| Anelosimus chickeringi Herbert Walt..., 1956 | Cobweb spiders |
| Anelosimus jucundus (Octavius Pic..., 1896) | ⇒ Anelosimus tosus Ralph Vary C..., 1916 |
| Anelosimus studiosus (Nicholas Mar..., 1850) | ⇒ Anelosimus fraternus Agnarsson, 2006 |
| Argiope argentata Fabricius, 1775 | Banded Garden Spider |
| Argiope submaronica (Fabricius, 1775) | ⇒ Argiope argentata Fabricius, 1775 |
| Argyrodes argyrodes (Walckenaer, 1842) | ⇒ Neospintharus trigonum Nicholas Mar..., 1850 |
| Ariadna isthmica Joseph A. Be..., 1970 | |
| Balmaceda turneri Arthur Merto..., 1946 | Jumping spiders |
| Beata rustica Peckham & Pe..., 1896 | Jumping spiders |
| Beata zeteki Arthur Merto..., 1946 | Jumping spiders |
| Bertrana striolata Keyserling, 1884 | Guinea ant |
| Breda milvina Carl Ludwig ..., 1846 | Jumping spiders |
| Breda notata Arthur Merto..., 1946 | Jumping spiders |
| Brignolia parumpunctata Simon, 1893 | |
| Camillina colon Norman I. Pl..., 1982 | Ground spiders |
| Castianeira cubana (Richard C. B..., 1926) | ⇒ Castianeira floridana Richard C. B..., 1904 |
| Castianeira dentata Arthur Merto..., 1937 | Corinnid sac spiders |
| Castianeira memnonia Carl Ludwig ..., 1841 | Corinnid sac spiders |
| Castianeira trimac Reiskind, 1969 | Corinnid sac spiders |
| Cesonia notata Chickering, 1949 | Ground spiders |
| Chrosiothes silvaticus Simon, 1894 | Cobweb spiders |
| Chrysso questona Herbert Walt..., 1962 | Cobweb spiders |
| Chrysso silva Herbert Walt..., 1962 | Cobweb spiders |
| Corythalia bicincta Alexander Pe..., 1925 | Jumping spiders |
| Corythalia obsoleta Richard C. B..., 1929 | Jumping spiders |
| Corythalia parvula (Peckham & Pe..., 1896) | ⇒ Anasaitis cubana Roewer, 1951 |
| Corythalia spiralis Frederick Oc..., 1901 | Jumping spiders |
| Costarina dura Chickering, 1951 | Goblin spiders |
| Costarina seclusa Chickering, 1951 | Goblin spiders |
| Costarina watina Chickering, 1968 | Goblin spiders |
| Cupiennius coccineus Frederick Oc..., 1901 | Red-legged Banana Spider |
| Cupiennius granadensis Eugen von Ke..., 1877 | Wandering spiders |
| Cybaeodamus meridionalis Lise, Ott &..., 2010 | |
| Cyrtognatha insolita Chickering, 1956 | Longjawed orb weavers |
| Dipoena atlantica Arthur Merto..., 1943 | Cobweb spiders |
| Dipoena duodecimpunctata Arthur Merto..., 1943 | Cobweb spiders |
| Dipoena zeteki Arthur Merto..., 1943 | Cobweb spiders |
| Dipoenata conica Arthur Merto..., 1943 | Cobweb spiders |
| Dysdera nomada Eugène Simon, 1910 | Stocky cerith |
| Enna nesiotes Chamberlin, 1925 | Long legged water spiders |
| Epicadus taczanowskii Roewer, 1951 | |
| Episinus dominicus Herbert Walt..., 1955 | |
| Episinus panamensis Herbert Walt..., 1955 | Cobweb spiders |
| Eriophora fuliginea C. L. Koch, 1838 | Orb weavers |
| Eustala bifida Frederick Oc..., 1904 | Orb weavers |
| Eustala conformans Ralph Vary C..., 1925 | Orb weavers |
| Eustala delecta Arthur Merto..., 1955 | Orb weavers |
| Eustala ingenua Arthur Merto..., 1955 | Orb weavers |
| Eustala oblonga Arthur Merto..., 1955 | Orb weavers |
| Eustala scitula Arthur Merto..., 1955 | Orb weavers |
| Eustala scutigera Octavius Pic..., 1898 | Orb weavers |
| Eustala sedula Arthur Merto..., 1955 | Orb weavers |
| Eustala semifoliata Octavius Pic..., 1899 | Orb weavers |
| Eustala tantula Arthur Merto..., 1955 | Orb weavers |
| Eustala vegeta Eugen von Ke..., 1865 | Orb weavers |
| Eustiromastix frontalis Banks, 1929 | |
| Freya justina Richard C. B..., 1929 | Jumping spiders |
| Freya prominens Frederick Oc..., 1901 | Jumping spiders |
| Gasteracantha cancriformis Linnaeus, 1758 | Crab spider |
| Gelanor zonatus C. L. Koch, 1845 | Pirate spiders |
| Glenognatha foxi McCook, 1894 | Longjawed orb weavers |
| Glenognatha spherella Chamberlin &..., 1936 | |
| Grammonota secata Arthur Merto..., 1970 | Sheet-web spiders |
| Grammonota teresta Arthur Merto..., 1970 | Sheet-web spiders |
| Hamataliwa barroana Ralph Vary C..., 1936 | Lynx spiders |
| Hamataliwa globosa Frederick Oc..., 1902 | Lynx spiders |
| Hamataliwa triangularis Otto Kraus, 1955 | Lynx spiders |
| Hesydrus palustris Eugène Simon, 1898 | Long legged water spiders |
| Heteropoda venatoria (Linnaeus, 1767) | ⇒ Heteropoda simplex Jäger & Ono, 2000 |
| Hogna chickeringi Ralph Vary C..., 1936 | Wolf spiders |
| Hypognatha testudinaria Władysław ..., 1879 | |
| Jollas geniculatus Eugène Simon, 1901 | Jumping spiders |
| Kukulcania hibernalis Hentz, 1842 | Crevice weaver |
| Lasaeola donaldi Arthur Merto..., 1943 | Cobweb spiders |
| Leptofreya bifurcata F.O.Pickard-..., 1901 | |
| Lycosa eutypa Ralph Vary C..., 1925 | Wolf spiders |
| Lyssomanes anchicaya María Elena ..., 1984 | Jumping spiders |
| Lyssomanes elegans Frederick Oc..., 1900 | Jumping spiders |
| Lyssomanes leucomelas Cândido Firm..., 1917 | |
| Lyssomanes patens Peckham & Pe..., 1896 | Jumping spiders |
| Mangora amchickeringi Levi, 2005 | Orb weavers |
| Mangora melanocephala Taczanowski, 1874 | Orb weavers |
| Martella bicavata Arthur Merto..., 1946 | Jumping spiders |
| Martella furva Arthur Merto..., 1946 | Jumping spiders |
| Metagonia panama Gertsch, 1986 | Cellar spiders |
| Metaphidippus fastosus Arthur Merto..., 1946 | Jumping spiders |
| Miagrammopes simus Ralph Vary C..., 1936 | Hackled orb weavers |
| Miagrammopes unipus Arthur Merto..., 1968 | Hackled orb weavers |
| Micrathena duodecimspinosa Octavius Pic..., 1890 | Orb weavers |
| Micrathena excavata Carl Ludwig ..., 1836 | Orb weavers |
| Micrathena flaveola Josef Anton ..., 1839 | Orb weavers |
| Micrathena furcula Octavius Pic..., 1890 | Orb weavers |
| Micrathena horrida Władysław ..., 1873 | Orb weavers |
| Micrathena schreibersi Josef Anton ..., 1833 | Orb weavers |
| Misumenoides rubrithorax Lodovico di ..., 1947 | |
| Misumenoides tibialis Octavius Pic..., 1891 | Crab spiders |
| Misumenops pallidus Berland, 1934 | Reticulated swellshark |
| Modisimus dilutus Gertsch, 1941 | |
| Modisimus pulchellus Banks, 1929 | |
| Myrmecotypus lineatipes Arthur Merto..., 1937 | Corinnid sac spiders |
| Myrmecotypus pilosus Octavius Pic..., 1898 | Corinnid sac spiders |
| Myrmecotypus rettenmeyeri Unzicker, 1965 | Corinnid sac spiders |
| Neoscona nautica L. Koch, 1875 | Orb weavers |
| Nephila clavipes (Linnaeus, 1767) | ⇒ Alpaida sulphurea Taczanowski, 1873 |
| Noegus spiralifer Frederick Oc..., 1901 | Jumping spiders |
| Nops largus Arthur M. Ch..., 1967 | |
| Nops ursumus Arthur M. Ch..., 1967 | |
| Novafrontina uncata Frederick Oc..., 1902 | |
| Nycerella delecta Peckham & Pe..., 1896 | Jumping spiders |
| Nycerella donaldi Arthur Merto..., 1946 | Jumping spiders |
| Ocrepeira galianoae Herbert Walt..., 1993 | |
| Oecobius concinnus Eugène Simon, 1893 | Disc web spiders |
| Olios antiguensis Eugen von Ke..., 1880 | |
| Oonopoides pallidulus Chickering, 1951 | Goblin spiders |
| Oonops persitus Arthur M. Ch..., 1970 | Goblin spiders |
| Opopaea deserticola Eugène Simon, 1891 | |
| Pachomius bilobatus F.O.Pickard-..., 1901 | |
| Pachomius dybowskii Taczanowski, 1871 | Jumping spiders |
| Pachomius ministerialis C.L.Koch, 1846 | |
| Parachemmis fuscus Arthur Merto..., 1937 | Corinnid sac spiders |
| Paradossenus isthmus Carico & Silva, 2010 | Long legged water spiders |
| Pelegrina bicuspidata Frederick Oc..., 1901 | Jumping spiders |
| Peucetia rubrolineata Eugen von Ke..., 1877 | Lynx spiders |
| Phiale bulbosa Frederick Oc..., 1901 | Jumping spiders |
| Phiale geminata Frederick Oc..., 1901 | Jumping spiders |
| Philoponella republicana Simon, 1891 | Hackled orb weavers |
| Philoponella semiplumosa Simon, 1893 | Hackled orb weavers |
| Philoponella tingens Chamberlin &..., 1936 | Hackled orb weavers |
| Philoponella vittata Keyserling, 1881 | Hackled orb weavers |
| Phoneutria boliviensis F.O. P.-Camb..., 1897 | Wandering spiders |
| Phycosoma altum Eugen von Ke..., 1886 | Cobweb spider |
| Phylloneta pictipes Keyserling, 1884 | |
| Pirata turrialbicus Alfred Russe..., 1978 | Wolf spider |
| Pronous intus Herbert Walt..., 1995 | Orb weavers |
| Pronous shanus Herbert Walt..., 1995 | Orb weavers |
| Psecas euoplus Ralph Vary C..., 1936 | Jumping spiders |
| Pseudanapis gertschi Raymond Robe..., 1958 | |
| Rabidosa rabida Walckenaer, 1837 | Rabid Wolf Spider |
| Scaphiella barroana Willis J. Ge..., 1941 | Goblin spiders |
| Scopocira dentichelis Eugène Simon, 1900 | |
| Scytodes championi (F. O. P.-Cam..., 1899) | ⇒ Scytodes zamorano Brescovit & ..., 2001 |
| Scytodes diminuta Valerio, 1981 | Spitting spiders |
| Scytodes guttipes Simon, 1893 | |
| Scytodes panamensis Brescovit & ..., 2001 | Spitting spiders |
| Selenops mexicanus Eugen von Ke..., 1880 | Wall crab spiders |
| Senoculus iricolor Simon, 1880 | |
| Sidusa incurva Chickering, 1946 | Jumping spiders |
| Sidusa mandibularis G.W.Peckham ..., 1896 | Jumping spiders |
| Sidusa pallida Frederick Oc..., 1901 | Jumping spiders |
| Simonestus separatus Joachim Schmidt, 1971 | Corinnid sac spiders |
| Sisicottus panopeus František M..., 1999 | |
| Sparianthina selenopoides Richard C. B..., 1929 | Huntsman spiders |
| Spintharus flavidus Nicholas Mar..., 1850 | Cobweb spiders |
| Stemmops cambridgei Herbert Walt..., 1955 | Cobweb spiders |
| Stemmops questus Herbert Walt..., 1955 | |
| Synotaxus turbinatus Eugène Simon, 1895 | |
| Tapinillus longipes Władysław ..., 1872 | Lynx spiders |
| Testudinaria elegans Władysław ..., 1879 | Delicate giant-turris |
| Tetragnatha mexicana Eugen von Ke..., 1865 | Longjawed orb weavers |
| Teudis cordobensis Cândido Firm..., 1941 | |
| Thaumasia heterogyna Ralph Vary C..., 1936 | Nursery web spiders |
| Thaumasia senilis Josef Anton ..., 1833 | Kingston dewberry |
| Theridiosoma zygops Chamberlin &..., 1936 | Ray spiders |
| Tidarren mixtum (Octavius Pic..., 1896) | ⇒ Tidarren haemorrhoidale Philip Bertkau, 1880 |
| Tmarus productus Arthur Merto..., 1950 | Crab spiders |
| Trachelas barroanus (Ralph Vary C..., 1925) | ⇒ Trachelas triangulus Norman I. Pl..., 1974 |
| Trachelas bispinosus Frederick Oc..., 1899 | Corinnid sac spiders |
| Trachelas ecudobus Arthur Merto..., 1972 | Corinnid sac spiders |
| Trachelas panamanus Arthur Merto..., 1937 | Corinnid sac spiders |
| Trachelas prominens Norman I. Pl..., 1974 | Corinnid sac spiders |
| Trachelas triangulus Norman I. Pl..., 1974 | Corinnid sac spiders |
| Trechalea extensa Octavius Pic..., 1896 | Long legged water spiders |
| Triaeris stenaspis Simon, 1891 | Goblin Spiders |
| Triggella infuscata F.O.Pickard-..., 1901 | |
| Uaiuara barroana Chamberlin, 1925 | Merced Canyon shoulderband |
| Uloborus glomosus Charles Atha..., 1842 | Feather-legged Spider |
| Wagneriana taboga Herbert Walt..., 1991 | Digger Bee |
| Wamba crispulus Eugène Simon, 1895 | |
| Wendilgarda mustelina Simon, 1897 | |
| Xanthofreya albosignata F.O.Pickard-..., 1901 | |
| Xanthofreya arraijanica Chickering, 1946 | |
| Zuniga magna Peckham & Pe..., 1892 | Jumping spiders |
| Zygoballus electus Arthur Merto..., 1946 | Jumping spiders |
| Zygoballus incertus Richard C. B..., 1929 | Jumping spiders |
| Zygoballus optatus Chickering, 1946 | Jumping spiders |
