| Megachile (Acentron) albitarsis Cresson, 1872 | White-footed Leaf-cutter Bee |
| Megachile (Amegachile) hilli Cockerell, 1929 | |
| Megachile (Argyropile) parallela Smith, 1853 | Parallel Leaf-cutter Bee |
| Megachile (Argyropile) rossi Mitchell, 1943 | |
| Megachile (Argyropile) sabinensis Mitchell, 1934 | |
| Megachile (Argyropile) townsendiana Cockerell, 1898 | |
| Megachile (Austromegachile) trigonaspis Schrottky, 1913 | |
| Megachile (Callomegachile) sculpturalis Smith, 1853 | Giant resin bee |
| Megachile (Callomegachile) umbripennis Smith, 1853 | Shadow-winged Resin Bee |
| Megachile (Chalicodoma) murina Friese, 1913 | |
| Megachile (Chalicodoma) sicula (Rossi, 1792) | ⇒ Megachile nasidens Friese, 1898 |
| Megachile (Chelostomoides) adelphodonta Cockerell, 1924 | |
| Megachile (Chelostomoides) angelarum Cockerell, 1902 | |
| Megachile (Chelostomoides) armaticeps Cresson, 1869 | |
| Megachile (Chelostomoides) axyx Snelling, 1990 | |
| Megachile (Chelostomoides) browni Mitchell, 1934 | |
| Megachile (Chelostomoides) campanulae Robertson, 1903 | Bellflower resin bee |
| Megachile (Chelostomoides) chilopsidis Cockerell, 1900 | Desert-willow Resin Bee |
| Megachile (Chelostomoides) davidsoni Cockerell, 1902 | Golden-eardrops Resin Bee |
| Megachile (Chelostomoides) discorhina Cockerell, 1924 | |
| Megachile (Chelostomoides) exilis Cresson, 1872 | Slender Resin Bee |
| Megachile exilis parexilis Mitchell, 1937 | |
| Megachile (Chelostomoides) georgica Cresson, 1878 | |
| Megachile (Chelostomoides) lobatifrons Cockerell, 1924 | |
| Megachile (Chelostomoides) manni Mitchell, 1934 | |
| Megachile (Chelostomoides) occidentalis Fox, 1894 | |
| Megachile (Chelostomoides) odontostoma Cockerell, 1924 | |
| Megachile (Chelostomoides) otomita Cresson, 1878 | |
| Megachile (Chelostomoides) prosopidis (Cockerell, 1900) | ⇒ Megachile occidentalis Fox, 1894 |
| Megachile (Chelostomoides) rugifrons Smith, 1854 | Rugose-Fronted Resin Bee |
| Megachile (Chelostomoides) spinotulata Mitchell, 1934 | |
| Megachile (Chelostomoides) subexilis Cockerell, 1908 | |
| Megachile (Chelostomoides) texensis Mitchell, 1956 | |
| Megachile (Creightonella) atrata Smith, 1853 | Stout tagelus |
| Megachile (Creightonella) frontalis Fabricius, 1804 | Hairy cockle |
| Megachile (Cressoniella) zapoteca Cresson, 1878 | |
| Megachile (Dasymegachile) chilensis (Spinola, 1851) | ⇒ Megachile saulcyi Guérin-Ménev..., 1845 |
| Megachile (Dasymegachile) saulcyi Guérin-Ménev..., 1845 | |
| Megachile (Eutricharaea) apicalis (Spinola, 1808) | ⇒ Megachile analis Nylander, 1852 |
| Megachile (Eutricharaea) chrysopyga Smith, 1853 | Stout tagelus |
| Megachile (Eutricharaea) concinna Smith, 1853 | Elegant leaf-cutter bee |
| Megachile (Eutricharaea) diligens (Smith, 1879) | ⇒ Megachile dentitarsus Sladen, 1919 |
| Megachile (Eutricharaea) fullawayi Cockerell, 1914 | Leafcutter bee |
| Megachile (Eutricharaea) gratiosa Smith, 1853 | |
| Megachile (Eutricharaea) leachella Curtis, 1828 | Megachile dorsalis |
| Megachile (Eutricharaea) obtusa Smith, 1853 | Stout tagelus |
| Megachile (Eutricharaea) pictiventris Smith, 1879 | Bunchberry dogwood |
| Megachile (Eutricharaea) rotundata Fabricius, 1787 | Alfafa leafcutting bee |
| Megachile (Eutricharaea) semiflava (Cockerell, 1937) | ⇒ Megachile cordata Smith, 1879 |
| Megachile (Eutricharaea) seychellensis Cameron, 1907 | |
| Megachile (Eutricharaea) simplex (Smith, 1853) | ⇒ Megachile inermis Provancher, 1888 |
| Megachile (Eutricharaea) timberlakei Cockerell, 1920 | Leaf-cutter bee |
| Megachile (Gronoceras) felina Gerstäcker, 1857 | |
| Megachile (Hackeriapis) apicata Smith, 1853 | Stout tagelus |
| Megachile (Hackeriapis) horatii Cockerell, 1913 | |
| Megachile (Hackeriapis) ignita Smith, 1853 | |
| Megachile (Hackeriapis) tosticauda Cockerell, 1912 | |
| Megachile (Leptorachis) petulans Cresson, 1878 | Petulant Leaf-cutter Bee |
| Megachile (Litomegachile) brevis (Say, 1837) | ⇒ Megachile pseudobrevis Mitchell, 1934 |
| Megachile brevis pseudobrevis Mitchell, 1935 | |
| Megachile (Litomegachile) coquilletti Cockerell, 1915 | |
| Megachile (Litomegachile) gentilis Cresson, 1872 | Leafcutter bee |
| Megachile (Litomegachile) lippiae Cockerell, 1900 | |
| Megachile (Litomegachile) mendica Cresson, 1878 | Flat-tailed Leaf-cutter Bee |
| Megachile (Litomegachile) onobrychidis Cockerell, 1908 | Western Little Leaf-cutter Bee |
| Megachile (Litomegachile) pankus Bzdyk, 2012 | |
| Megachile (Litomegachile) pseudobrevis Mitchell, 1934 | Southeastern Little Leaf-cutter Bee |
| Megachile (Litomegachile) snowi (Mitchell, 1927) | ⇒ Megachile mendica Cresson, 1878 |
| Megachile (Litomegachile) texana Cresson, 1878 | Texas Leaf-cutter Bee |
| Megachile (Megachile) centuncularis Linnaeus, 1758 | Common leaf-cutter bee |
| Megachile (Megachile) inermis Provancher, 1888 | Freckled milkvetch |
| Megachile (Megachile) lapponica Thomson, 1872 | Alfilaree |
| Megachile (Megachile) ligniseca Kirby, 1802 | Wood-Carving Leaf-Cutter Bee |
| Megachile (Megachile) montivaga Cresson, 1878 | Silver-tailed Petal-cutter Bee |
| Megachile (Megachile) nivalis (Friese, 1903) | ⇒ Megachile lapponica Thomson, 1872 |
| Megachile (Megachile) relativa Cresson, 1878 | Golden-Tailed Leafcutter |
| Megachile (Megachiloides) alamosana (Mitchell, 1934) | ⇒ Megachile anograe Cockerell, 1908 |
| Megachile (Megachiloides) alata Mitchell, 1934 | |
| Megachile (Megachiloides) amica Cresson, 1872 | |
| Megachile (Megachiloides) anograe Cockerell, 1908 | |
| Megachile (Megachiloides) brimleyi Mitchell, 1926 | |
| Megachile (Megachiloides) casadae Cockerell, 1898 | |
| Megachile (Megachiloides) coloradensis (Mitchell, 1936) | ⇒ Megachile zapoteca Cresson, 1878 |
| Megachile (Megachiloides) dakotensis Mitchell, 1926 | |
| Megachile (Megachiloides) deflexa Cresson, 1878 | |
| Megachile (Megachiloides) fucata Mitchell, 1934 | |
| Megachile (Megachiloides) gravita Mitchell, 1934 | |
| Megachile (Megachiloides) hookeri Cockerell, 1915 | |
| Megachile (Megachiloides) integra Cresson, 1878 | |
| Megachile (Megachiloides) integrella Mitchell, 1926 | |
| Megachile (Megachiloides) legalis Cresson, 1879 | |
| Megachile (Megachiloides) manifesta Cresson, 1878 | |
| Megachile (Megachiloides) mucorosa Cockerell, 1908 | |
| Megachile (Megachiloides) nevadensis Cresson, 1879 | |
| Megachile (Megachiloides) oenotherae Mitchell, 1924 | |
| Megachile (Megachiloides) pascoensis Mitchell, 1934 | |
| Megachile (Megachiloides) rubi Mitchell, 1924 | |
| Megachile (Megachiloides) soledadensis Cockerell, 1900 | |
| Megachile (Megachiloides) subnigra Cresson, 1879 | |
| Megachile (Megachiloides) wheeleri Mitchell, 1927 | |
| Megachile (Megachiloides) wyomingensis Mitchell, 1934 | |
| Megachile (Megachiloides) xerophila Cockerell, 1933 | |
| Megachile (Melanosarus) bahamensis Mitchell, 1927 | Bahama Leaf-cutter Bee |
| Megachile (Melanosarus) xylocopoides Smith, 1853 | Carpenter-mimic Leaf-cutter Bee |
| Megachile (Neochelynia) chichimeca Cresson, 1878 | |
| Megachile (Neochelynia) stomatura Cockerell, 1917 | |
| Megachile (Neocressoniella) carbonaria (Smith, 1853) | ⇒ Megachile gemula Cresson, 1878 |
| Megachile (Neoeutrichaea) rotundata Fabricius, 1793 | Alfalfa leaf-cutter bee |
| Megachile (Paracella) coelostoma (Cockerell, 1932) | ⇒ Megachile barbata Smith, 1853 |
| Megachile (Pseudocentron) azteca Cresson, 1878 | |
| Megachile (Pseudocentron) poeyi Guérin-Ménev..., 1845 | |
| Megachile (Pseudocentron) pollinosa Spinola, 1851 | |
| Megachile (Pseudocentron) pruina Smith, 1853 | Hoary Leaf-cutter Bee |
| Megachile (Pseudocentron) sidalceae Cockerell, 1897 | |
| Megachile (Pseudocentron) terrestris (Schrottky, 1902) | ⇒ Megachile mellitarsis Cresson, 1878 |
| Megachile (Pseudomegachile) ericetorum Lepeletier, 1841 | Banded Mud Bee |
| Megachile (Pseudomegachile) lanata Fabricius, 1775 | Wooly Wall Bee |
| Megachile (Pseudomegachile) nigripes Spinola, 1838 | Megachilid bee |
| Megachile (Sayapis) fidelis Cresson, 1878 | Faithful leafcutting bee |
| Megachile (Sayapis) frugalis Cresson, 1872 | |
| Megachile (Sayapis) inimica Cresson, 1872 | Hostile Leaf-cutter Bee |
| Megachile inimica sayi Cresson, 1878 | |
| Megachile (Sayapis) newberryae Cockerell, 1900 | Newberry´s leaf-cutter Bee |
| Megachile (Sayapis) policaris Say, 1831 | |
| Megachile (Sayapis) pugnata Say, 1837 | Pugnacious Leaf-cutter Bee |
| Megachile (Schizomegachile) monstrosa Smith, 1868 | |
| Megachile (Xanthosarus) addenda Cresson, 1878 | Cranberry Leafcutter |
| Megachile (Xanthosarus) agustini Cockerell, 1905 | |
| Megachile (Xanthosarus) analis Nylander, 1852 | |
| Megachile (Xanthosarus) circumcincta Kirby, 1802 | Black-headed leaf-cutter bee |
| Megachile (Xanthosarus) cochisiana Mitchell, 1934 | |
| Megachile (Xanthosarus) comata Cresson, 1872 | |
| Megachile (Xanthosarus) dentitarsus Sladen, 1919 | |
| Megachile (Xanthosarus) fortis Cresson, 1872 | |
| Megachile (Xanthosarus) frigida Smith, 1853 | Frigid Leaf-cutter Bee |
| Megachile (Xanthosarus) gemula Cresson, 1878 | Small-handed Leaf-cutter Bee |
| Megachile (Xanthosarus) giliae (Cockerell, 1906) | ⇒ Megachile circumcincta Kirby, 1802 |
| Megachile (Xanthosarus) ingenua Cresson, 1878 | |
| Megachile (Xanthosarus) innupta Cockerell, 1915 | |
| Megachile (Xanthosarus) latimanus Say, 1823 | Broad-handed Leaf-cutter Bee |
| Megachile (Xanthosarus) melanophaea Smith, 1853 | Black-and-gray Leaf-cutter Bee |
| Megachile (Xanthosarus) mucida Cresson, 1878 | |
| Megachile (Xanthosarus) perihirta Cockerell, 1898 | Furry Leaf-cutter Bee |
| Megachile (Xanthosarus) willughbiella (Kirby, 1802) | ⇒ Megachile willughbiella Kirby, 1802 |
| Megachile abducta Mitchell, 1926 | |
| Megachile albula Lovell & Coc..., 1907 | |
| Megachile angelica Mitchell, 1933 | |
| Megachile angonica Cockerell, 1937 | |
| Megachile angularis Mitchell, 1930 | |
| Megachile angusta Mitchell, 1930 | |
| Megachile aspera Mitchell, 1924 | |
| Megachile astata Mitchell, 1933 | |
| Megachile astragali Mitchell, 1938 | |
| Megachile avara Cresson, 1878 | |
| Megachile bakeri (Cockerell, 1918) | ⇒ Megachile pascoensis Mitchell, 1934 |
| Megachile binominata Smith, 1853 | Stout tagelus |
| Megachile binotulata Dalla Torre, 1896 | |
| Megachile bishoppi Cockerell, 1915 | |
| Megachile boharti Mitchell, 1942 | |
| Megachile bradleyi Mitchell, 1934 | |
| Megachile bruneri Mitchell, 1934 | |
| Megachile bucephala (Fabricius, 1793) | ⇒ Megachile pugnatus Say, 1837 |
| Megachile calogaster Cockerell, 1898 | |
| Megachile chelostomoides Gribodo, 1894 | |
| Megachile chlorura Cockerell, 1918 | Leafcutter bee |
| Megachile chomskyi Sheffield, 2013 | |
| Megachile chrysothamni Cockerell, 1908 | |
| Megachile clara Mitchell, 1930 | |
| Megachile cleomis (Cockerell, 1900) | ⇒ Megachile texana Cresson, 1878 |
| Megachile cordata Smith, 1879 | Bunchberry dogwood |
| Megachile coreopsana Mitchell, 1933 | |
| Megachile cylindrica Friese, 1906 | |
| Megachile decipiens Lovell & Coc..., 1907 | |
| Megachile denticulata (Reiche & Fai..., 1847) | ⇒ Megachile lanata Fabricius, 1775 |
| Megachile dentipes (Vachal, 1909) | ⇒ Megachile cylindrica Friese, 1906 |
| Megachile disparilis Cresson, 1872 | |
| Megachile disparipennis W.P.Cockerell, 1917 | |
| Megachile dorsata Smith, 1853 | Stout tagelus |
| Megachile dulciana Mitchell, 1934 | |
| Megachile duplexa Mitchell, 1933 | |
| Megachile emoryi Cockerell, 1904 | |
| Megachile empeyi Pasteels, 1970 | |
| Megachile exclamans Viereck, 1916 | |
| Megachile facunda Cresson, 1872 | |
| Megachile fastidiosa Mitchell, 1930 | |
| Megachile florensis Mitchell, 1943 | |
| Megachile fuerteventurae Tkalcu, 1993 | |
| Megachile fulvohirta Friese, 1904 | |
| Megachile gabrielensis Mitchell, 1933 | |
| Megachile geneana Gribodo, 1894 | |
| Megachile generosa Cresson, 1878 | |
| Megachile grandis Cresson, 1872 | |
| Megachile hilata Mitchell, 1934 | |
| Megachile howardi Cockerell, 1919 | |
| Megachile impartita Mitchell, 1934 | |
| Megachile indianorum Cockerell, 1913 | |
| Megachile infragilis Cresson, 1878 | |
| Megachile ingens Friese, 1903 | |
| Megachile instita Mitchell, 1934 | |
| Megachile inyoensis Mitchell, 1942 | |
| Megachile kallstroemiae Cockerell, 1908 | |
| Megachile konowi | |
| Megachile laticeps Smith, 1853 | Stout tagelus |
| Megachile laurita Mitchell, 1927 | |
| Megachile lucrosa Cresson, 1872 | |
| Megachile macleayi Cockerell, 1907 | |
| Megachile macneilli Mitchell, 1957 | |
| Megachile manumuskin Viereck, 1902 | |
| Megachile maurata Mitchell, 1936 | |
| Megachile megagyna Cockerell, 1909 | |
| Megachile melanderi Mitchell, 1944 | |
| Megachile melanogaster Eversmann, 1852 | |
| Megachile mellitarsis Cresson, 1878 | |
| Megachile meriani (Strand, 1911) | ⇒ Megachile seychellensis Cameron, 1907 |
| Megachile micheneri Mitchell, 1936 | |
| Megachile mojavensis Mitchell, 1934 | |
| Megachile montonii Gribodo, 1894 | |
| Megachile morio (Smith, 1853) | ⇒ Megachile pruina Smith, 1853 |
| Megachile moschata Mitchell, 1933 | |
| Megachile murinella Cockerell, 1908 | |
| Megachile neglecta Mitchell, 1924 | |
| Megachile nelsoni Mitchell, 1936 | |
| Megachile newelli Cockerell, 1908 | |
| Megachile nupta Cresson, 1872 | |
| Megachile optiva Cresson, 1872 | |
| Megachile oslari Mitchell, 1934 | |
| Megachile osmioides Cresson, 1872 | |
| Megachile pacifica (Panzer, 1798) | ⇒ Megachile rotundata Fabricius, 1787 |
| Megachile pallorea Vachal, 1903 | |
| Megachile palmensis Mitchell, 1934 | |
| Megachile pararhodura Cockerell, 1910 | |
| Megachile pararubi Mitchell, 1957 | |
| Megachile paratexana Mitchell, 1930 | |
| Megachile parksi Mitchell, 1936 | |
| Megachile penicillata Cockerell, 1915 | |
| Megachile perbrevis Cresson, 1878 | |
| Megachile perniciosa (Friese, 1903) | ⇒ Megachile rufiventris Guérin-Ménev..., 1834 |
| Megachile perplexa Smith, 1853 | |
| Megachile pinguis Cresson, 1878 | |
| Megachile praedicta ♰ T. D. A. Coc..., 1908 | |
| Megachile praefica (Gribodo, 1894) | ⇒ Megachile saulcyi Guérin-Ménev..., 1845 |
| Megachile pratti Cockerell, 1913 | |
| Megachile pruinosa (Friese, 1903) | ⇒ Megachile texana Cresson, 1878 |
| Megachile pseudolegalis Mitchell, 1957 | |
| Megachile pseudonigra Mitchell, 1927 | |
| Megachile pusilla Perez, 1884 | |
| Megachile redlandica Mitchell, 1933 | |
| Megachile relicta Cockerell, 1913 | |
| Megachile rhyssalus Wu, 2005 | |
| Megachile sapellonis Cockerell, 1900 | |
| Megachile sayi Cresson, 1878 | |
| Megachile schismatura Cockerell, 1908 | |
| Megachile scotti (Cockerell, 1937) | ⇒ Megachile wahlbergi Friese, 1901 |
| Megachile secunda (Dalla Torre, 1896) | ⇒ Megachile pictiventris Smith, 1879 |
| Megachile semilaurita Mitchell, 1927 | |
| Megachile seraxensis Radoszkowski, 1893 | |
| Megachile shermani Mitchell, 1924 | |
| Megachile smithi Mitchell, 1933 | |
| Megachile stoddardensis Mitchell, 1957 | |
| Megachile studiosa (Bingham, 1899) | ⇒ Megachile exilis Cresson, 1872 |
| Megachile subanograe Mitchell, 1934 | |
| Megachile sublaurita Mitchell, 1927 | |
| Megachile tepaneca Cresson, 1878 | |
| Megachile tephrosiana Mitchell, 1927 | |
| Megachile toluca Cresson, 1878 | |
| Megachile toscata Mitchell, 1934 | |
| Megachile tucsonensis Mitchell, 1956 | |
| Megachile umatillensis Mitchell, 1927 | |
| Megachile victoriana Mitchell, 1934 | |
| Megachile virginiana Mitchell, 1926 | |
| Megachile wahlbergi Friese, 1901 | |
| Megachile yumensis Mitchell, 1944 | |
| Megachile zaptlana Cresson, 1878 | |
